脑电分析系列[MNE-Python-20]| 脑电图处理和事件相关电位(ERP)

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脑电分析系列[MNE-Python-20]| 脑电图处理和事件相关电位(ERP)

2021-08-12
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今天主要介绍一下MNE-Python中进行脑电图处理和事件相关电位(ERP)。

有后台留言问，代码是在哪里运行的。这里说明一下，案例介绍的代码均在jupyter notebook中运行的，当然这些代码也可以在PyCharm等IDE中运行(不过可能存在在不同环境下代码需要稍微改动的情况。)

Python脑电图处理案例：


import mne
from mne.datasets import sample


# 加载数据文件
data_path = sample.data_path()
raw_fname = data_path + '/MEG/sample/sample_audvis_filt-0-40_raw.fif'
event_fname = data_path + '/MEG/sample/sample_audvis_filt-0-40_raw-eve.fif'
# 这些EEG数据已经默认有平均参考(average reference)
raw = mne.io.read_raw_fif(raw_fname, preload=True)


# 只筛选 EEG 和 EOG数据
raw.pick_types(meg=False, eeg=True, eog=True)
 
 
# 查看 raw 信息， 可以发现有59个EEG通道和1个EOG通道
print(raw.info)

实际上，拥有一些EOG通道的EEG通道是很普遍的。在实际获取EEG数据时，会受到眼动等活动的影响。

如果要更改通道类型，可以使用mne.io.Raw.set_channel_types()方法。
例如，要将EOG通道视为EEG，可以使用以下方法更改其类型：


raw.set_channel_types(mapping={'EOG 061': 'eeg'})
print(raw.info)

比较上面前后两个打印中的红框内容，可以发现，EOG已被EEG取代了。


# 更改EOG通道的名称
raw.rename_channels(mapping={'EOG 061': 'EOG'})
 
 
# 为了讲述案例，我们将EOG频道重设为EOG类型。
raw.set_channel_types(mapping={'EOG': 'eog'})


"""
样本数据集中的EEG通道有对应通道位置。这些位置可在每个通道说明的"位置"中找到。
下面是获取第一个通道的位置
"""
 
 
print(raw.info['chs'][0]['loc'])

[-0.03737009  0.10568011  0.07333875


  0.00235201  0.11096951 -0.03500458
  0.   1.   0.    0.    0.    1. ]


"""
可以使用mne.io.Raw.plot_sensors()绘制通道位置。
如果您的数据没有位置，则可以使用MNE随附的Montages来设置
"""
raw.plot_sensors()

raw.plot_sensors('3d')  # in 3D

设置脑电图参考

首先，从原始对象中删除参考。

这显示的移除了MNE默认的EEG平均参考。

raw_no_ref, _ = mne.set_eeg_reference(raw, [])

EEG channel type selected for re-referencing

EEG data marked as already having the desired reference. Preventing automatic future re-referencing to an average reference.

Removing existing average EEG reference projection.

然后，定义了Epochs，并计算了左听觉状态的ERP。


reject = dict(eeg=180e-6, eog=150e-6)
event_id, tmin, tmax = {'left/auditory': 1}, -0.2, 0.5
events = mne.read_events(event_fname)
epochs_params = dict(events=events, event_id=event_id, tmin=tmin, tmax=tmax,
                     reject=reject)
 
 
evoked_no_ref = mne.Epochs(raw_no_ref, **epochs_params).average()
del raw_no_ref  # save memory
 
 
title = 'EEG Original reference'
evoked_no_ref.plot(titles=dict(eeg=title), time_unit='s')
evoked_no_ref.plot_topomap(times=[0.1], size=3., title=title, time_unit='s')

设置平均参考电极


"""
平均参考：通常默认情况下添加，但也可以显式添加。
"""
 
 
raw.del_proj()
raw_car, _ = mne.set_eeg_reference(raw, 'average', projection=True)
evoked_car = mne.Epochs(raw_car, **epochs_params).average()
del raw_car  # save memory
 
 
title = 'EEG Average reference'
evoked_car.plot(titles=dict(eeg=title), time_unit='s')
evoked_car.plot_topomap(times=[0.1], size=3., title=title, time_unit='s')

自定义参考：使用通道EEG 001和EEG 002的平均值作为参考


raw_custom, _ = mne.set_eeg_reference(raw, ['EEG 001', 'EEG 002'])
evoked_custom = mne.Epochs(raw_custom, **epochs_params).average()
del raw_custom  # save memory
 
 
title = 'EEG Custom reference'
evoked_custom.plot(titles=dict(eeg=title), time_unit='s')
evoked_custom.plot_topomap(times=[0.1], size=3.,
                           title=title, time_unit='s')

可以使用' / '分隔的'标记'来选择Epochs中的试验子集。

首先，我们创建一个包含4个条件的Epochs对象。


event_id = {'left/auditory': 1, 'right/auditory': 2,
            'left/visual': 3, 'right/visual': 4}
epochs_params = dict(events=events, event_id=event_id, tmin=tmin, tmax=tmax,
                     reject=reject)
epochs = mne.Epochs(raw, **epochs_params)
 
 
print(epochs)

接下来，我们创建左刺激和右刺激试验的平均值。

我们可以使用基本的操作，例如，构建和绘制不同的ERP。


left, right = epochs["left"].average(), epochs["right"].average()
 
 
# create and plot difference ERP
joint_kwargs = dict(ts_args=dict(time_unit='s'),
                    topomap_args=dict(time_unit='s'))
mne.combine_evoked([left, -right], weights='equal').plot_joint(**joint_kwargs)


    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EEG : ['EEG 001']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']

这是权重相等的差异。如果试验编号不平衡，则也可以考虑使每个条件的事件数相等（使用epochs.equalize_event_counts）。例如，首先，我们为每种情况创建单独的ERP。


aud_l = epochs["auditory", "left"].average()
aud_r = epochs["auditory", "right"].average()
vis_l = epochs["visual", "left"].average()
vis_r = epochs["visual", "right"].average()
 
 
all_evokeds = [aud_l, aud_r, vis_l, vis_r]
print(all_evokeds)


"""
这可以通过Python列表理解来简化
"""
 
 
all_evokeds = [epochs[cond].average() for cond in sorted(event_id.keys())]
print(all_evokeds)
 
 
# 然后，我们也以这种方式构造和绘制左右试验的未加权平均值：
mne.combine_evoked(
    [aud_l, -aud_r, vis_l, -vis_r], weights='equal').plot_joint(**joint_kwargs)


    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EEG : ['EEG 001']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
    Rejecting  epoch based on EOG : ['EOG']
['left/auditory' (average, N=56), [-0.1998, 0.49949] sec, 59 ch, ~3.1 MB>, 'left/visual' (average, N=67), [-0.1998, 0.49949] sec, 59 ch, ~3.1 MB>, 'right/auditory' (average, N=62), [-0.1998, 0.49949] sec, 59 ch, ~3.1 MB>, 'right/visual' (average, N=56), [-0.1998, 0.49949] sec, 59 ch, ~3.1 MB>]

通常，在字典或列表中存储诱发对象是有意义的——无论是不同的条件，还是不同的主题。


"""
如果将它们存储在一个列表中，就可以很容易地对它们求平均值，例如，跨主题(或条件)的总平均值。
"""
grand_average = mne.grand_average(all_evokeds)
mne.write_evokeds('tmp\\tmp-ave.fif', all_evokeds)
 
 
# 如果“诱发对象”对象存储在词典中，则可以按名称检索它们。
all_evokeds = dict((cond, epochs[cond].average()) for cond in event_id)
print(all_evokeds['left/auditory'])
 
 
# 除了显式访问外，还可以用于设置标题。
for cond in all_evokeds:
    all_evokeds[cond].plot_joint(title=cond, **joint_kwargs)

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